In today's era, it is a necessity to pay attention to local and cultural abilities in media productions. The increasing development of educational media has considered the need to pay more attention to their localization. The aim of the present research was to compile and validate the native content standards of educational multimedia. In this study, an exploratory sequential combination method was used. The statistical population was related to the qualitative part of all books and scientific research articles. The number of these sources was determined based on data sufficiency. And in the quantitative section, there were experts who were somehow related to this research. The research sample in the quantitative part included 61 experts who were selected by the snowball sampling method. After reviewing library documents and articles, a list of primary content criteria for native educational multimedia was obtained. This list consisted of 21 items whose formal and content validity was checked and confirmed by 5 experts. Then, in the factor analysis section, the factor load of the items was calculated by collecting the opinions of 61 sample experts. For the inferential analysis of the data, Lawshe's reliability coefficient and exploratory factor analysis and confirmatory factor analysis were used. Using exploratory and confirmatory factor analysis, out of 21 existing items, 19 items remained for the native content criteria of educational multimedia. The results showed that 19 criteria can be considered for the content design of educational multimedia. According to local needs and capacities in the design and production of educational multimedia, the approved criteria can be considered and used in the design and production of local educational multimedia.